Retina International's Scientific Newsletter - Small Nucleotide-binding Proteins

	Retina International's Scientific Newsletter
Editor's Notes from literature on Small GTP-binding Proteins (RAB, RAS)

Recent update from: 03.01.2000

Rab

Notes

Rab 5 regulates endosome-endosome recognition in early endosomes
endosome recognition is depending on N-terminus
Rab5 is the only trypsin cleaved polypeptide at low trypsin conditions
hypervariable C-terminus
interaction with a GAP protein

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (20)

Rab

Notes

Rab2: pre-Golgi-membranes
Rab4: early endosomes
Rab6: sends Rhodopsin-vesicles to plasma membrane and returns to Golgi apparatus detached from membrane

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (7)

Rab

Notes

Rab8: associated with post-Golgi-membranes and actin

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (21)

Ras

Notes

Modified with 3 types of lipids: C16 palmitoyl, C14 myristoyl, C15/C20 prenyl (farnesyl/geranyl)
16C defective forms are not able to associate with membrane and couple less effective to their targets

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (4)

Rab

Notes

post Golgi: Rab 3, 6, 8
trans Golgi network/post Golgi: Rab 11
Rab 11 sublocalization = Rab 6
Retention of RHO in Rab depleted vesicles

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (8)

Rab

Notes

Low molecular weight proteins GTP binding
Adhere to surface of cell membranes by virtue of covalently attached geranylgeranyl at COOH terminal Cys
Geranylated by GGtase II
Rab1A: endoplasmatic reticulum
Rab3A: neuronal vesicals
Rab5A: fusion of plasma membrane

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (5)

Rab, ras

Notes

Rab 5 terminates CC-SN
Rab 11 terminates CC-Gln-Asn-Ile (CCAAX)
Both are geranylated
Rab 5 is no substrate for GGtase I
AAX is removed by a specific membrane-bound endopeptidase after prenylation
the exposed COOH terminus is subjected to methylation by microsomal membrane-bound methyltransferase
ras proteins are farnesylated
Non-processed ras are able to interact with a molecule of the ras-mediated signaling

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (4)

Rab

Notes

Rab5 is geranylated with 2 moles of geranylgeranyl per mole Rab5

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (15)

Rab

Notes

Inactive GDP-bound form, active GTP-bound form
Activity promoted by GEF, GAP, and GDI
Peripheral membrane protein
Soluble as GDP-GDI-Rab 80 kDa delivery complex

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (25)

Rab

Notes

Rab1A ER->Golgi->intra Golgi
mutations -> disassembly of Golgi stacks
BFA -> GTP exchange protein for Rab
membrane arrival from ER to Golgi is blocked

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (24)

Rab

Notes

Geranylation is influenced by nucleotide status
Likely to be GTP-bound after synthesis
Possibly GTPase-activating protein enhances interaction to REP1
4 regions of Rabs proposed to be involved in REP1 binding:
- Amino-terminal region including conserved Lys
- Effector domain loop2/b2 region
- Loop3/b3 region
- Carboxy-terminal region
Rab-GTPase rates are extremely slow

Species:

See also: REP1

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (17)

Rab

Notes

3 Factors:
1. Guanine nucleotide release factor (GRF)
  - Puts GDP out, GTP in
  - GRF3A known
2. Rab GDP dissociation inhibitor (GDI)
3. GDP-exchange activating protein (GAP)
  - GAP3A and GAP6 characterized
GDI for a number of Rab, complex with prenylated Rab
CXC or CC motif N-terminal

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (2)

Rab

Notes

Rab functions

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (11)

Rab, 1

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 7p or 2p
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 1

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 11

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 10

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 2p24-p23
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 10

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 12 proximal

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 11

Notes

On TGN-derived vesicles of constitutive and regulated secretory pathways
pI~6
Distribution parallels Rab6

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (6)

Rab, 11b-rs1

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 1q32-q41
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 11b-rs1

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 1

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 11b-rs2

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 6p21/21q22
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 11b-rs2

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 17

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 17

Notes

Expressed in polarized and non-polarized cells
Epithelial cell specific
In kidney and intestine
In intestine apical and basolateral in epithelial cells
In kidney in vesicular structures in the apical domain of the proximal tubule cells

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 1

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (10)

Rab, 17

Notes

Expressed in polarized and non-polarized cells
Epithelial cell specific
In kidney, liver, small intestine
Induced in mesenchymal precursors upon differentiation
Localized to the plasmamembrane of the basolateral domain
In kidney in vesicular structures in the apical domain of the proximal tubule cells

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 1

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (10)

Rab, 1b

Notes

Is bound by REP preferentially in the GDP state
Substitutions I73N, Y78D, A81D in the a2 helix (Switch 2 region) of Rab1B prevents prenylation
Switch 2 region is responsible for selective interatcion with REP
Conformation of Switch 2 is depending on the GDP/GTP bound
I73N, Y78D have decreased GTPase activity
Comparably D44N, I41N in Switch 1 domain show reduced prenylation
SWITCH 2 domain of Rab27 is 55% homologues to Rab1B

Species: unspecified

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (12)

Rab, 22

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 20q13
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 22

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 2 distal

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 24

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 5q
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 24

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 13 proximal

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 27

Notes

Rab 27 is REP1 specific

Species: Homo sapiens

See also: REP1

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE: 27 kDa
	calculated:

Reference: (16)

Rab, 27

Notes

Rab27 is escorted to prenylation by REP1
Purification scheme for Rab27
Unknown function
Expression in RPE and choriocapillaris
Unprenylated Rab27 remains cytosolic and is inactive
GG-Rab27 binds GTP and attaches to organelle
GG-Rab27 hydrolyses GTP to GDP on organelle fusion
GG-Rab27-GDP is resolubilized by GDI, which exchanges GDP for GTP and leads GG-Rab27 back to the organelle
40% identical to Rab3 family, but represents distinct family
Cytosolic in CHM

Species: Homo sapiens

See also: REP1

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers: To obtain full length cDNA

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (16)

Rab, 27

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE: 27 kDa
	calculated:

Reference: (19)

Rab, 27

Notes

predominantly membrane bound
distinct subfamily
40% identical to Rab3
low affinity in REP2/Rab27 interaction

Species: Homo sapiens

See also: REP1

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons: 1

Primers: complete

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (16)

Rab, 27a

Notes

maps near BBS4

Species: Homo sapiens

See also: REP1

Chromosomal localisation	in man: 15q22.3-23
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (18)

Rab, 3a

Notes

pI~4
On RHO-bearing post-Golgi vesicles
Most labelling of Rab3a associated with structures outside the synapse
Negatively controls Ca²⁺-dependent exocytosis

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (6)

Rab, 3a

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 19p13.2
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (13)

Rab, 3a

Notes

30% homology to ras gene product
Thermolabile
Trypsin sensitive
Regulatory proteins: GRF and GAP3a specific

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE: 25 kDa
	calculated:

Reference: (3)

Rab, 3b

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 1p31-p32
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (14)

Rab, 4a

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 1q42-q43
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 4a

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 1q42-q43
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 4a

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 8

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 4b

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 19q13
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 4b

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 7 proximal

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 5

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 3p24-p22
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 5, 7

Notes

Isoprenoid moiety derived from mevalonate
Blocking of intracellular mevalonate resulted in four-fold increase of Rab5 and Rab7 protein levels
Rab5/Rab7 increase indicate regulation of expression by isoprenoid pathway

Species:

See also: REP1

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (9)

Rab, 6

Notes

In trans-Golgi membranes and TGN
IR near the cilium in ROS
Megative ragulator of anterograde intra-Golgi transport
GTP-bound forms induce the redistribution of Golgi proteins into the ER in HeLa cells
pI~5

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE: 24 kDa
	calculated: 23.5 kDa

Reference: (6)

Rab, 6

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 2q14-q21
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 7

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 11q or 15q
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 7

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 9 central

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (1)

Rab, 8

Notes

Regulates polarized membrane transport to the basolateral membrane in epithelial cells and dendrites in neurons
Common to the post-Golgi vesicles and ROS membranes
pI~5
Not associated with the photoreceptor Golgi
Colocalizes with actin filaments and RHO-bearing post Golgi vesicles near the base of the cilium
RHO-bearing post-Golgi vesicle are enriched in Rab8
Possible link between the cytoskeleton and polarized membrane traffic

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE: 23 kDa
	calculated:

Reference: (6)

Rab, 8

Notes

Ends in CVLL => substrate for GGTaseI
REP-induced conformational change on escorting is not essential for exposure of hat e Rab C-terminal cysteine prenylation site
Majority of Rab8 is prenylated by GGTaseII
On binding to REP target recognition is not dependent on a specific Cys arrangement at the C-terminus

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (23)

Rab, 8

Notes

Ends CVLL
Serves as substrate for REP/GGTase II and GGTase I in cell free assay
Y78D mutant does not serve for GGTase II, its prenylation is reduced 60 - 70% in intact cells
Major geranylation established by REP/GGTase II system
Geranylation is dependent on recognition outside the geranylation motif

Species:

See also: GGTase II

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated:

Reference: (23)

rho A, rho A,B,C

Notes

Light-dependent ADP-ribosylation
90% homology to each other
Involved in regulation of cell proliferation and differentiation by affecting the cytoskeletal system
Active in GTP-bound form
Regulated by GAP, GDI, and GDS
Pertussis toxin C3 substrates are not removed from membranes on illumination

Species: Bos taurus

See also: RHO

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Amino acids:

Molecular weight	on SDS-PAGE:
	calculated: 20 - 30 kDa

Reference: (22)

References:

1. Barbosa, M.D., Johnson, S.A., Achey, K., Gutierrez, M.J., Wakeland, E.K., Zerial, M., and Kingsmore, S.F. The Rab protein family: Genetic mapping of six Rab genes in the mouse. 1996; Genomics. 439 - 444.
Goto Top

2. Beranger, F., Cadwallader, K., Porfiri, E., Powers, S., Evans, T., de Gunzburg, J., and Hancock, J.F. Determination of structural requirements for the interaction of Rab6 with RabGDI and Rab geranylgeranyltransferase. 1994; J.Biol.Chem. 269: 13637 - 13643.
Goto Top

3. Burstein, E.S. and Macara, I.G. Characterization of a guanine nucleotide-releasing factor and a GTPase-activating protein that are specific for the ras-related protein p25rab3A. 1992; Proc.Natl.Acad.Sci.U.S.A. 89: 1154 - 1158.
Goto Top

4. Casey, P.J., Moomaw, J.F., Zhang, F.L., Higgins, J.B., and Thissen, J.A. Prenylation and G protein signaling. 1994; Recent.Prog.Horm.Res. 49: 215 - 238.
Goto Top

5. Cremers, F.P., Armstrong, S.A., Seabra, M.C., Brown, M.S., and Goldstein, J.L. REP-2, a Rab escort protein encoded by the choroideremia-like gene. 1994; J.Biol.Chem. 269: 2111 - 2117.
Goto Top

6. Deretic, D. Rab proteins and post-Golgi trafficking of rhodopsin in photoreceptor cells. 1997; Electrophoresis. 18: 2537 - 2541.
Goto Top Link to PudMed

7. Deretic, D., Huber, L.A., Ransom, N., Mancini, M., Simons, K., and Papermaster, D.S. Small G-protein Rab8 may participate in rdos disk morphogenesis in photoreceptors. 1994; Invest.Ophthalmol.Vis.Sci. 34: 2151
Goto Top

8. Deretic, D., Puleo Schepke, B., and Trippe, C. Formation of rhodopsin-bearing post-golgi vesicles in a cell free system. 1995; Invest.Ophthalmol.Vis.Sci. 36: S513
Goto Top

9. Laezza, C., Bucci, C., Santillo, M., Bruni, C.B., and Bifulco, M. Control of Rab5 and Rab7 expression by the isoprenoid pathway. 1998; Biochem.Biophys.Res.Commun. 248: 469 - 472.
Goto Top Link to PudMed

10. McMurtrie, E.B., Barbosa, M.D., Zerial, M., and Kingsmore, S.F. Rab17 and rab18, small GTPases with specificity for polarized epithelial cells: genetic mapping in the mouse. 1997; Genomics. 45: 623 - 625.
Goto Top Link to PudMed

11. Nuoffer, C. and Balch, W.E. GTPases: Multifunctional molecular switches regulating vesicular traffic. 1994; Ann.Rev.Biochem. 63: 949 - 990.
Goto Top

12. Overmeyer, J.H., Wilson, A.L., Erdman, R.A., and Maltese, W.A. The putative "switch 2" domain of the Ras-related GTPase, Rab1B, plays an essential role in the interaction with Rab escort protein. 1998; Mol.Biol.Cell. 9: 223 - 235.
Goto Top Link to PudMed

13. Rousseau Merck, M.F., Zahraoui, A., Bernheim, A., Touchot, N., Miglierina, R., Tavitian, A., and Berger, R. Chromosome mapping of the human RAS related Rab3A and Rab3B genes to chromosomes 19p13.2 and 1p31-p32 respectively. 1989; Cytogenet.Cell.Genet. 51: 1070
Goto Top

14. Rousseau Merck, M.F., Zahraoui, A., Bernheim, A., Touchot, N., Miglierina, R., Tavitian, A., and Berger, R. Chromosome mapping of the human ras-related rab3A gene to 19p13.2. 1989; Genomics. 5: 694 - 698.
Goto Top

15. Sanford, J.C., Pan, Y., and Wessling Resnick, M. Prenylation of Rab5 is dependent on guanine nucleotide binding. 1993; J.Biol.Chem. 268: 23773 - 23776.
Goto Top

16. Seabra, M.C. The pathogenesis of choroideremia: identification of a REP 1 specific Rab. 1995; Invest.Ophthalmol.Vis.Sci. 36: S893
Goto Top

17. Seabra, M.C. Nucleotide dependence of Rab geranylgeranylation. Rab escort protein interacts preferentially with GDP-bound Rab. 1996; J.Biol.Chem. 271: 14398 - 14404.
Goto Top

18. Seabra, M.C., Anant, J.S., Desnoyers, L., and Burke, S. Molecular Pathogenesis Of Choroideremia: Kinetics Of REP-Rab Binding. 1997; Invest.Ophthalmol.Vis.Sci. 38: S680
Goto Top

19. Seabra, M.C., Ho, Y.K., and Anant, J.S. Deficient geranylgeranylation of Ram/Rab27 in choroideremia. 1995; J.Biol.Chem. 270: 24420 - 24427.
Goto Top Link to PudMed

20. Steele Mortimer, O., Clague, M.J., Huber, L.A., Chavrier, P., Gruenberg, J., and Gorvel, J.P. The N-terminal domain of a rab protein is involved in membrane-membrane recognition and/or fusion. 1994; EMBO Journal. 13: 34 - 41.
Goto Top

21. Tian, N. and Slaugther, M. The structure-activityanalysisof retinal on bipolar metabotropic glutamate receptor agonists. 1994; Invest.Ophthalmol.Vis.Sci. 35: 1567
Goto Top

22. Wieland, T., Ulibarri, I., Gierschik, P., Hall, A., Aktories, K., and Jakobs, K.H. Interaction of recombinant rho A GTP-binding proteins with photoexcited rhodopsin. 1990; FEBS Letters. 274: 111 - 114.
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23. Wilson, A.L., Erdman, R.A., Castellano, F., and Maltese, W.A. Prenylation of Rab8 GTPase by type I and type II geranylgeranyl transferases. 1998; Biochem.J. 333: 497 - 504.
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24. Wilson, B.S., Nuoffer, C., Meinkoth, J.L., McCaffery, M., Feramisco, J.R., Balch, W.E., and Farquhar, M.G. A Rab1 mutant affecting guanine nucleotide exchange promotes disassembly of the Golgi apparatus. 1994; J.Cell Biol. 125: 557 - 571.
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25. Wu, S.K., Zeng, K., Wilson, I.A., and Balch, W.E. Structural insights into the function of the Rab GDI superfamily. 1996; Trends.Biochem.Sci. 21: 472 - 476.
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