Retina International's Scientific Newsletter - Interphotoreceptor Retinoid-binding Protein

	Retina International's Scientific Newsletter
Editor's Notes from literature on Interphotoreceptor Retinoid-binding Protein (IRBP, RBP3)

Recent update from: 02.01.2000

RBP3, IRBP

Notes

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 146 kDa
	calculated:

Reference: (21)

RBP3, IRBP

Notes

in space between photoreceptorouter segments, on the apical surface of RPE

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (6)

RBP3, IRBP

Notes

negates involvement in RP
526 patients, ADRP, ARRP, RP simplex

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (24)

RBP3, IRBP

Notes

Arg267Gln, Val282Met, Ser291Arg, Asn785Lys, Arg833Cys in RAl Degenerations but no coseggregation
9 silent changes

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (17)

RBP3, IRBP

Notes

Binds predominantly 11cRAl and atRAl

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (7)

RBP3, IRBP

Notes

Only protein to remove efficient RAl from RPE membranes
IRBP lipid transfer is not RPE membrane specific
2 Rol binding sites

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (1)

RBP3, IRBP

Notes

Promoter: -156 -> +19
ATTAA -42 -> -76
retinoic acid responsive element -85 -> -105 AP2 sites

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (11)

RBP3, IRBP

Notes

inhibitory cis element -1173 -> -350
enhancing cis element -350 -> -204

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (4)

RBP3, IRBP

Notes

Predominant protein of IPM
IRBP affinity to Rol is 100 times less than CRBP affinity
In RPE Rol is associated to CRBP
IRBP increases processing of Rol to 11cRAl, increases the release of 11cRAl from the RPE
Promotes regeneration of RHO in bleached ROS
Possibly: [CRBP] contributes to Rol flow from ROS to RPE und processing of Ry, IRBP may facilitate processing of Rol to 11cRAl as a RAl sink in IPM, IRBP may protect retinoids

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 133 kDa
	calculated:

Reference: (10)

RBP3, IRBP

Notes

Shuttles retinoids between the retinal pigment epithelium and ROS
Binds cis- and trans isomers of Rol and Ral, RA, vitamin E, long-chain fatty acids
Delivers 11-cis-RAl
2 binding sites
70% of bound fatty acids = palmitic, oleic, and stearic acid
DHA showed only slight effect on atRol binding at site 1 and less efefct on 11-cis Ral binding
11-cis Ral dissociation is profoundly affected by DHA

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 140 kDa
	calculated:

Reference: (28)

RBP3, IRBP

Notes

R1005Q doubles the amount of ligand necessary to attain saturation by increasing the number of binding sites
K_d was not changed
R1041G, R1073G, and R1122G have no effect on K_d and binding sites
Mutations do not change the ligand specificity

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (2)

Online Reference

RBP3, IRBP

Notes

Species: Bos taurus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (3)

RBP3, IRBP

Notes

Association of IRBP and metalloproteinases (gelatinase and caseinase)
Possibly contamination?

Species: Bos taurus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (22)

RBP3, IRBP Notes

Retinois are bound by repeat 2 and 3
Repeat is nor required for binding

Species: Bos taurus See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data
Exons:
Primers:
Protein Data Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (23)

RBP3, IRBP

Notes

Half the size as mammalian RHO
cDNA of 6.2 kb

Species: Danio rerio

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (27)

RBP3, IRBP

Notes

mRNA detected 24 hpf in pineal and 50 hpf in ventral retina
Cones express IRBP before the opsin, rods express rhodopsin before IRBP
Ventronasal quadrant is the first to express IRBP

Species: Danio rerio

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (26)

Online Reference

RBP3, IRBP

Notes

Species: Gallus gallus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

mRNA: 1.3 kb

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 140 kDa
	calculated:

Reference: (19)

RBP3, IRBP

Notes

Each repeat posses the ability to bind 9cRAl, atRol, atRA, DHA, a- and b-ionine, and trans-parinaic acid
Each repeat retains all functions of the whole protein
Repeats 1 and 2 correspond to a deep site, repeats 3 and 4 correspond to a shallow binding site
Binding occurs specific with all repeats and does not cooperativity having its own saturability and affinity

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (18)

RBP3, IRBP

Notes

Binds 2 Rol

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons: 4

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (16)

RBP3, IRBP

Notes

Binds hydrophobic ligands in the retina
4 repeats of 300 AA
Each repeat posses the capability to bind Rol and 16-anthyloxy-palmitic acid
Binds and transports 11cRAl from stores in the RPE to the photoreceptor
Glycosylated in insect expression systems
Fatty acid analogs bind more tigthly to IRBP than retinoids

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons: 4

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (13)

RBP3, IRBP

Notes

4 homologue segments, 300 AA each
Transports RAl and Rol from and to regeneration
Binds 11cRol, retinoic acid, cholesterol, fatty acids and tocopherol
single subunit
BglII und StyI RFLP

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 10p11.2-q11.2
	in mouse:

Gene Data

cDNA:

Exons:

Primers:

Protein Data

Aminoacids: 1230 AA

Molecularweight	on SDS-PAGE: 133 kDa
	calculated:

Reference: (15)

RBP3, IRBP

Notes

IRBP receptor on cell surface (70 kDa)

Species: Monkey

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (9)

RBP3, IRBP

Notes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 15

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (8)

RBP3, IRBP

Notes

IRBP promoter studies
DNAseI protection between -69 - -4, identical in bovine and mouse

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (14)

RBP3, IRBP

Notes

Methylated promotor in mouse and cow at MspI site

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (5)

RBP3, IRBP

Notes

IRBP promoter studies
Heterogenous activity throughout the retinae
Confined to photoreceptor cells
Activity in rod and cones
mRNA in photoreceptor and pineal gland
1,3 kb 5� fragment sufficient to direct tissue specific expression to photoreceptor cells
IRBP promotor is photoreceptorspecific
Expression starts at embryonic day 17

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (29)

RBP3, IRBP

Notes

IRBP promoter studies
Promotor segment -156 - +19 confers tissue specificity
Equals human -123 - +18 region
Only 70 bp 5' transcription start are required for promotor activity
-70 - -45 two photoreceptor specific elements RET-1/PCE1 and its reversed repeat(GATTAA-box)
GATTAA-box binds CRX-element
beyond -70 is an element that inhibits expression in neurons

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (29)

RBP3, IRBP

Notes

Borst-box 90% homologue in man and bovine

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (18)

Online Reference

RBP3, IRBP

Notes

in rd mice IRBP is mainly intracellular
IRBP is secreted by the RPE
Do endogenous factors regulate secretion?
No abnormal production in rd mice

Species: Mus musculus rd

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 140 kDa
	calculated:

Reference: (25)

RBP3, IRBP

Notes

IRBP is internalized by RPE via endocytosis and phagocytosis
Phagocytosis inhibition at constant light inhibited IRBP specific activity
Endocytosis plays an important role in removal of IRBP from IPM

Species: Xenopus laevis

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (20)

RBP3, IRBP

Notes

Synthezsized by the neural retina and secreted into IPM by rods and cones
Facilitates the exchange of 11cRAl and atRAl
Major soluble component of IPM
May have arisen from quadruplication of a single gene
Identity to human 70%

Species: Xenopus laevis

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

mRNA: 4.2 kb

Exons:

Primers:

Protein Data

Aminoacids: 293 AA

Molecularweight	on SDS-PAGE: 124 kDa
	calculated:

Reference: (12)

References:

1. Adler, A.J. and Edwards, R.B. IRBP Uniquely Removes 11 Retinal from Isolated RPE Membranes. 1995; Invest.Ophthalmol.Vis.Sci. 36: S6
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2. Baer, C.A., van Niel, E.E., Cronk, J.W., Kinter, M.T., Sherman, N.E., Braiman, M.S., and Gonzalez-Fernandez, F. Arginine to glutamine substitutions in the fourth module of Xenopus interphotoreceptor retinoid-binding protein. 1998; Mol.Vis. 4: 30
Goto Top Link to PudMed
Human Mutation Online

3. Barrett, D.J., Redmond, T.M., Wiggert, B., Oprian, D.D., Chader, G.J., and Nickerson, J.M. cDNA clones encoding bovine interphotoreceptor retinoid binding protein. 1985; Biochem.Biophys.Res.Comm. 131: 1086 - 1093.
Goto Top

4. Boatright, J.H., Borst, D.E., Lin, Z.Y., Si, J.S., Bhati, M., Patel, D., Bruno, J., and Nickerson, J.M. Functional Characterization of Cis Acting Regulatory Elements of the IRBP Gene Promotor Region. 1995; Invest.Ophthalmol.Vis.Sci. 36: S123
Goto Top

5. Borst, D.E. DNA methylation of the IRBP promotor. 1994; Invest.Ophthalmol.Vis.Sci. 34: 1703
Goto Top

6. Bunt Milam, A.H. and Saari, J.C. Immunocytochemical localization of two retinoid-binding proteins in vertebrate retina. 1983; J.Cell Biol. 97: 703 - 712.
Goto Top Link to PudMed

7. Chen, Y. and Noy, N. Retinoid specificity of interphotoreceptor retinoid-binding protein. 1994; Biochemistry. 33: 10658 - 10665.
Goto Top Link to PudMed

8. Danciger, M., Kozak, C.A., Nickerson, J., Redmond, T.M., and Farber, D.B. Localization of the gene for interphotoreceptor retinoid-binding protein to mouse chromosome 14 near Np-1. 1990; Genomics. 8: 727 - 731.
Goto Top

9. Duncan, T., Palmer, K., Chader, G.J., and Wiggert, B. A Putative Retinal Pigment Cell Surface Receptor for Interphotoreceptor Retinoid Binding Protein (IRBP). 1995; Invest.Ophthalmol.Vis.Sci. 36: S122
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10. Edwards, J.F., Storts, R.W., Joyce, J.R., Shelton, J.M., and Menzies, C.S. Juvenile-onset neuronal ceroid-lipofuscinosis in Rambouillet sheep. 1994; Vet.Pathol. 31: 48 - 54.
Goto Top Link to PudMed

11. Fei, Y., Matragoon, S., and Liou, G.I. Characterization of the interphotoreceptor retinoid binding protein (IRBP) gene promotor. 1995; Invest.Ophthalmol.Vis.Sci. 36: S123
Goto Top

12. Gonzalez Fernandez, F., Kittredge, K.L., Rayborn, M.E., Hollyfield, J.G., Landers, R.A., Saha, M., and Grainger, R.M. Interphotoreceptor retinoid-binding protein (IRBP), a major 124 kDa glycoprotein in the interphotoreceptor matrix of Xenopus laevis. Characterization, molecular cloning and biosynthesis. 1993; Journal.of.Cell Science. 105: 7 - 21.
Goto Top

13. Lin, Z.Y., Li, G.R., Phong, M., Si, J.S., Takizawa, N., and Nickerson, J.M. Study Of Recombinant Human IRBP And Its Mutations. 1997; Invest.Ophthalmol.Vis.Sci. 38: A1 - A1000.
Goto Top

14. Liou, G.I. Localization and identification of a retina-specific cis-element in the gene encoding Interphotoreceptor Retinoid-binding Protein (IRBP). 1993; Invest.Ophthalmol.Vis.Sci. 34: 830
Goto Top

15. Liou, G.I., Ma, D.P., Yang, Y.W., Geng, L., Zhu, C., and Baehr, W. Human interstitial retinoid-binding protein. Gene structure and primary structure. 1989; J.Biol.Chem. 264: 8200 - 8206.
Goto Top Link to PudMed

16. McGee, T.L., Berson, E.L., and Dryja, T.P. Search for point mutations in the interstitial retinol-binding protein gene in patients with hereditary retinal degenerations. 1992; Invest.Ophthalmol.Vis.Sci. 33 (Suppl.): 1396
Goto Top

17. McGee, T.L., DeStefano, J.D., Berson, E.L., and Dryja, T.P. A complete screen of the entire coding sequence of the IRBP gene for mutations in patients with hereditary degenerations. 1993; Invest.Ophthalmol.Vis.Sci. 34: 1460
Goto Top

18. Nickerson, J.M., Li, G.R., Lin, Z.Y., Takizawa, N., Si, J.S., and Gross, E.A. Structure-function relationships in the four repeats of human interphotoreceptor retinoid-binding protein (IRBP). 1998; Mol.Vis. 4:33: 33
Goto Top Link to PudMed

19. Oliver, E.R., Cunningham, L.L., van Niel, E.E., Youssri, A.I., and Gonzalez Fernandez, F. Molecular cloning and characterization of chicken Interphotoreceptor Retinoid-binding Protein. 1997; Invest.Ophthalmol.Vis.Sci. 38: S304
Goto Top

20. Payman, R. and Gonzalez Fernandez, F. Turnover of IRBP in the Xenopus interphotoreceptor matrix. 1995; Invest.Ophthalmol.Vis.Sci. Suppl. S6
Goto Top

21. Pfeffer, B., Wiggert, B., Lee, L., Zonnenberg, B., Newsome, D., and Chader, G. The presence of a soluble interphotoreceptor retinol-binding protein (IRBP) in the retinal interphotoreceptor space. 1983; J.Cell Physiol. 117: 333 - 341.
Goto Top Link to PudMed

22. Plantner, J.J., Quinn, T.A., and Luo, X. Metalloproteinases Associated With Interphotoreceptor Retinoid Binding Protein. 1996; Invest.Ophthalmol.Vis.Sci. S909
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23. Redmond, T.M. and Nickerson, J.M. Retinoid binding to a recombinant central two-repeat segment of IRBP. 1992; Invest.Ophthalmol.Vis.Sci. 33: 1181
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24. Ringens, P.J., Fang, M., Shinohara, T., Bridges, C.D., Lerea, C.L., Berson, E.L., and Dryja, T.P. Analysis of genes coding for S-antigen, interstitial retinol binding protein, and the alpha-subunit of cone transducin in patients with retinitis pigmentosa. 1990; Invest.Ophthalmol.Vis.Sci. 31: 1421 - 1426.
Goto Top Link to PudMed

25. Smith, S.B., Lee, L., Nickerson, J., Si, J.S., Chader, G.J., and Wiggert, B. Synthesis and secretion of interphotoreceptor retinoid-binding protein (IRBP) and developmental expression of IRBP mRNA in normal and rd mouse retinas. 1992; Exp.Eye Res. 54: 957 - 963.
Goto Top

26. Stenkamp, D.L., Cunningham, L.L., Raymond, P.A., and Gonzalez-Fernandez, F. Novel expression pattern of interphotoreceptor retinoid-binding protein (IRBP) in the adult and developing zebrafish retina and RPE. 1998; Mol.Vis. 4:26: 26
Goto Top Link to PudMed

27. van Niel, E.E., Rajendran, R.R., and Gonzalez Fernandez, F. Zebafish IRBP gene. 1995; Invest.Ophthalmol.Vis.Sci. 36: S122
Goto Top

28. Wolf, G. Transport of retinoids by the interphotoreceptor retinoid-binding protein. 1998; Nutr.Rev. 56: 156 - 158.
Goto Top Link to PudMed

29. Yokoyama, T., Liou, G.I., Caldwell, R.B., and Overbeek, P.A. Photoreceptor-specific activity of the human interphotoreceptor retinoid-binding protein (IRBP) promoter in transgenic mice. 1992; Exp.Eye Res. 55: 225 - 233.
Goto Top Link to PudMed

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