Retina International's Scientific Newsletter

	Retina International's Scientific Newsletter
Editor's Notes from literature on Cone-Rod Homeobox Gene (CRX)

Recent update from: 19.12.1999

CRX

Notes

97% identical to mouse ortholog
Abundantly expressed in retina
Next relatives OTX1 and OTX2
Homeodomain from Glu 42 to Arg 98
Major regulator of outer segment proteins
WSP- motif between Asp 289 and Lys 294

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 19q13.3
	in mouse:

Gene Data

mRNA: 4.5 kb

Exons: 3

Primers: Complete + sequencing

Protein Data

Aminoacids: 299 AA

Molecularweight	on SDS-PAGE:
	calculated: 32 kDa

Reference: (1)

CRX

Notes

Binds to opsin promotor
Restricted to photoreceptors
R41 forms hydrogenbond to E80

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (3)

Crx

Notes

Expression restricted to mature and developing photoreceptors
Binds and transactivates TAATCC/A upstream several photoreceptor genes
Dominant negative form of Crx fails to form proper POS
High degree of homology to Otx1, Otx2
Homologue to Drosophila otd
OTX-tail: DPLDYKDQSAWK (not in Drosophila)
First detected signal at E12.5
Recognizes promotor of RHO, IRBP, SAG, OPS
Crx expression is not sufficient to instruct rod cell fate

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

mRNA: 3.0 kb

Exons: 3

Primers: Complete + sequencing

Protein Data

Aminoacids: 299 AA

Molecularweight	on SDS-PAGE:
	calculated: 32 kDa

Reference: (2)

References:

1. Freund, C.L., Gregory Evans, C.Y., Furukawa, T., Papaioannou, M., Looser, J., Ploder, L., Bellingham, J., Ng, D., Herbrick, J.A., Duncan, A., Scherer, S.W., Tsui, L.C., Loutradis Anagnostou, A., Jacobson, S.G., Cepko, C.L., Bhattacharya, S.S., and McInnes, R.R. Cone-rod dystrophy due to mutations in a novel photoreceptor-specific homeobox gene (CRX) essential for maintenance of the photoreceptor. 1997; Cell. 91: 543 - 553.
Goto Top Link to PudMed

2. Furukawa, T., Morrow, E.M., and Cepko, C.L. Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation. 1997; Cell. 91: 531 - 541.
Goto Top Link to PudMed

3. Weng, J., Belecky Adams, T., Adler, R., and Travis, G.H. Identification of two rds/peripherin homologs in the chick retina. 1998; Invest.Ophthalmol.Vis.Sci. 39: 440 - 443.
Goto Top Link to PudMed

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Contact the editor: irpamp@irpa.org	This site is maintained and edited by Dr. rer. medic. Markus Preising, Dipl.Biol. Molecular Genetics Laboratory Department of Paediatric Ophthalmology, Strabismology and Ophthalmogenetics University of Regensburg Head: Prof. Dr. med. Birgit Lorenz