Retina International's Scientific Newsletter - Cellular Retinol-binding Protein

	Retina International's Scientific Newsletter
Editor's Notes from literature on Cellular Retinol-binding Protein (CRBP)

Recent update from: 18.12.1999

CRBP

Notes

Binds reversible atRol
In liver, lung, spleen, brain, testes, ovaries and uterus

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 16 kDa
	calculated:

Reference: (1)

CRBP

Notes

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 14,8 kDa
	calculated:

Reference: (10)

CRBP

Notes

CRBP transports Rol from storage (liver) to target
CRBP II: Rol uptake from jejunum

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (9)

CRBP

Notes

Most atRol is bound by CRBP
Rol bound to CRBP cannot be channeled to the active site of ADH
[Rol] bound to CRBP intracellular is ~2 - 5 microM
CRBP is utilized by RDH1 and RDH2
Kd of CRBP ~0.1 nM Rol

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (6)

CRBP1, I

Notes

90,5% similarity to human
maximum 2 copies per genome
single mRNA

Species: Rattus norvegicus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

mRNA: 700 bp

Exons:

Primers:

Protein Data

Aminoacids: 134 AA

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (12)

CRBP1, type 1

Notes

Transports Rol to specific acceptor sites in nucleus
Single copy

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (8)

CRBP1, type 1

Notes

Specific intracellular Rol transport protein

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 3
	in mouse:

Gene Data

cDNA:

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (3)

CRBP1, type 1

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

cDNA:

Exons: 4

Primers:

Protein Data

Aminoacids: 134 AA,

Molecularweight	on SDS-PAGE: 15,7 kDa
	calculated:

Reference: (9)

CRBP1, type 1

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 3q21-q22
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (11)

CRBP1, type 1

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 3
	in mouse:

Gene Data

cDNA:

Exons:

Primers:

Protein Data

Aminoacids: 134 AA

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (2)

CRBP1, type 1

Notes

In many tissues of the body
IR restricted to cytoplasm and M�ller cell endfeet and radial processes
Transcytoplasmic carrier that accepts Rol from Rol-binding protein

Species: Rattus norvegicus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (5)

CRBP1, type 1

Notes

Species: Rattus norvegicus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids: 134 AA

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (13)

CRBP2, type 2

Notes

CRBP2 in jejunum

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (7)

CRBP2, type 2

Notes

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 3p11-qter
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (4)

CRBP2, type 2

Notes

Species: Rattus norvegicus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

cDNA:

Exons:

Primers:

Protein Data

Aminoacids: 134 AA

Molecularweight	on SDS-PAGE:
	calculated: 15580

Reference: (7)

References:

1. Bashor, M.M. and Chytil, F. Cellular retinol-binding protein. 1975; Biochim.Biophys.Acta. 411: 87 - 96.
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2. Colantuoni, V., Cortese, R., Nilsson, M., Lundvall, J., Bavik, C.O., Eriksson, U., Peterson, P.A., and Sundelin, J. Cloning and sequencing of a full length cDNA corresponding to human cellular retinol-binding protein. 1985; Biochem.Biophys.Res.Comm. 130: 431 - 439.
Goto Top

3. Colantuoni, V., Rocchi, M., Roncuzzi, L., and Romeo, G. Mapping of human cellular retinol-binding protein to chromosome 3. 1986; Cytogenet.Cell Genet. 43: 221 - 222.
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4. Demmer, L.A., Birkenmeier, E.H., Sweetser, D.A., Levin, M.S., Zollman, S., Sparkes, R.S., Mohandas, T., Lusis, A.J., and Gordon, J.I. The cellular retinol binding protein II gene. Sequence analysis of the rat gene, chromosomal localization in mice and humans, and documentation of its close linkage to the cellular retinol binding protein gene. 1987; J.Biol.Chem. 262: 2458 - 2467.
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5. Eisenfeld, A.J., Bunt Milam, A.H., and Saari, J.C. Localization of retinoid-binding proteins in developing rat retina. 1985; Exp.Eye Res. 41: 299 - 304.
Goto Top Link to PudMed

6. Kedishvili, N.Y., Gough, W.H., Davis, W.I., Parsons, S., Li, T.K., and Bosron, W.F. Effect of cellular retinol-binding protein on retinol oxidation by human class IV retinol/alcohol dehydrogenase and inhibition by ethanol. 1998; Biochem.Biophys.Res.Commun. 249: 191 - 196.
Goto Top Link to PudMed

7. Li, E., Demmer, L.A., Sweetser, D.A., Ong, D.E., and Gordon, J.I. Rat cellular retinol-binding protein II: use of a cloned cDNA to define its primary structure, tissue-specific expression, and developmental regulation. 1986; Proc.Natl.Acad.Sci.U.S.A. 83: 5779 - 5783.
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8. Liou, G.I., Fong, S.L., and Bridges, C.D. Comparison of cytosol retinol binding proteins from bovine retina, dog liver, and rat liver. 1981; J.Biol.Chem. 256: 3153 - 3155.
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9. Nilsson, M.H., Spurr, N.K., Lundvall, J., Rask, L., and Peterson, P.A. Human cellular retinol-binding protein gene organization and chromosomal location. 1988; Eur.J.Biochem. 173: 35 - 44.
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10. Ong, D.E. Purification and partial characterization of cellular retinol-binding protein from human liver. 1982; Cancer.Res. 42: 1033 - 1037.
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11. Rocchi, M., Covone, A., Romeo, G., Faraonio, R., and Colantuoni, V. Regional mapping of RBP4 to 10q23----q24 and RBP1 to 3q21----q22 in man. 1989; Somatic.Cell and.Molecular.Genetics. 15: 185 - 190.
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12. Sherman, D.R., Lloyd, R.S., and Chytil, F. Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA. 1987; Proc.Natl.Acad.Sci.U.S.A. 84: 3209 - 3213.
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13. Sundelin, J., Anundi, H., Tragardh, L., Eriksson, U., Lind, P., Ronne, H., Peterson, P.A., and Rask, L. The primary structure of rat liver cellular retinol-binding protein. 1985; J.Biol.Chem. 260: 6488 - 6493.
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Contact the editor: irpamp@irpa.org	This site is maintained and edited by Dr. rer. medic. Markus Preising, Dipl.Biol. Molecular Genetics Laboratory Department of Paediatric Ophthalmology, Strabismology and Ophthalmogenetics University of Regensburg Head: Prof. Dr. med. Birgit Lorenz