Retina International's Scientific Newsletter - Cellular Retinaldehyde-binding Protein

	Retina International's Scientific Newsletter
Editor's Notes from literature on Cellular Retinaldehyde-binding Protein (RLBP1, CRAlBP)

Recent update from: 18.12.1999

RLBP1, CRALBP

Notes

CRAlBP promoter: PCE1 at -243 -> +80
-1539 - 2089 region directs expression to RPE
No TATA
Additionally Sp1, AP2, NF, GT, GC-Box reversed, STDR

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (11)

RLBP1, CRALBP

Notes

transports 11cRAl/ 11cRol

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (18)

RLBP1, CRALBP

Notes

in RPE and Müller Cells

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (1)

RLBP1, CRALBP

Notes

Negates involvement in RP

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (4)

RLBP1, CRALBP

Notes

supports Pigment Epithelium Oxireductase with 11cis-retinoid

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (10)

RLBP1, CRALBP

Notes

Species:

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 33 kDa
	calculated:

Reference: (16)

RLBP1, CRALBP

Notes

about 3 nmol CRAlBP/adult Bovine eye
0,5% of soluble protein of retinal supernatant this is 1 nmol/retina and 1 nmol/RPE
sensitive to degradation by exogenous proteinases
degradation confined to NH-terminus
Purification scheme

Species: Bos taurus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated: 36000

Reference: (17)

RLBP1, CRALBP

Notes

interacts specific with 11-cis-RDH

Species: Bos taurus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

cDNA:

Exons:

Primers:

Protein Data

Aminoacids: 316 AA

Molecularweight	on SDS-PAGE:
	calculated: 36421

Reference: (7)

RLBP1, CRALBP

Notes

Carries 11cRAl and 11cRol
N- and C-terminus are immunodominant regions
atRAl is reduced to atRol inside photoreceptor
Watersoluble protein found only in retina and pineal gland
Remarkably stereoselective
Partition of 11cRol between oxidation or esterification is modulated by CRAlBP

Species: Bos taurus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

cDNA:

Exons:

Primers:

Protein Data

Aminoacids: 316 AA

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (6)

RLBP1, CRAlBP

Notes

Watersoluble protein
No alterations in USH1

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 15q26
	in mouse: 7

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 36 kDa
	calculated:

Reference: (20)

RLBP1, CRAlBP

Notes

Markers: D15S116, D15S154, D15S152, D15S1046

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 15q26
	in mouse:

Gene Data

Exons:

Primers: Exons 2 - 8

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (14)

RLBP1, CRAlBP

Notes

Minimal proteolytic fragment to bind RAl = AA 120 - 316
C-terminus involved in RA binding

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse: 7

Gene Data

Exons:

Primers:

Protein Data

Aminoacids: 316 AA

Molecularweight	on SDS-PAGE:
	calculated: 36347

Reference: (3)

RLBP1, CRAlBP

Notes

Excluded in Stargardt's disease

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons: 8

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (21)

RLBP1, CRALBP

Notes

Ry synthetase uses CRAlBP as substrate transporter

Species: Homo sapiens

See also:

Chromosomal localisation	in man: 15q26
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids: 316 AA

Molecularweight	on SDS-PAGE:
	calculated: 36378

Reference: (7)

RLBP1, CRALBP

Notes

DNA-potein interactions at -243 - -174 and -102 - -38
Contains PCE1 element

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (2)

RLBP1, CRALBP

Notes

Carries 11cRAl and/or 11cRol in RPE and retina (ratio 3:1)
Retinoid-binding domain in C-terminus
Bathochrome NMR-shift on binding of 9-cis-Ral

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (5)

RLBP1, CRALBP

Notes

Acts at the branch point of 11cRAl esterification and or oxidation by RDH
Q210 and K221 are components of the ligand binding pocket

Species: Homo sapiens

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (5)

RLBP1, CRALBP

Notes

14 kb genomic clone
Insert recognized by human CRAlBP probe
watersoluble protein
11cRAl saturated on isolation

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE: 36 kDa
	calculated:

Reference: (19)

Rlbp1, Cralbp

Notes

Abundantly expressed in the RPE and Müller cells
Positive regulatory activity between -2089 to -1539 and -243 to +80 bp
In the latter two PCE-1 sites
Human BCE not conserved in mice
CRX elelment in promotor
In Rat RPE expresse from E13 and in Müller cells from P1 on
Alu repetitive sequence between -1438 to -1157 bp
Repressor element between -702 to -243 bp
PCE-1 acts positive in RPE
Single copy gene
Exon 1 untranslated
CRAlBP promoter: PCE1 at -243 to +80
-1539 - 2089 region directs expression to RPE
Contains TATA-box
Additionally Sp1, AP2, NF, GT, GC-Box reversed, STDR
Nucleotide seqence identity to human: ~87% coding, ~65% non-coding

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse: 7

Gene Data

mRNA: 2.1

Exons: 8

Primers:

Protein Data

Aminoacids: 316 AA

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (12)

Online Reference

RLBP1, CRALBP

Notes

Ribozyme inhibition of IRBP function leads to reduced a- and b-wave amplitudes

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (13)

RLBP1, CRALBP

Notes

Mouse > 40% identity with human

Species: Mus musculus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

: 1,1 kb

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (9)

RLBP1, CRALBP

Notes

Positive immunoreactivity in Müller cells and RPE and CPE
Binds 11cRAl and 11cRol
CRAlBP to CRBP 7:1 in bovine retina

Species: Rattus norvegicus

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (8)

RLBP1, CRALBP

Notes

Regulatory elements Brn-2, MZF-1, AP-1, CRX

Species: recombinant

See also:

Chromosomal localisation	in man:
	in mouse:

Gene Data

Exons:

Primers:

Protein Data

Aminoacids:

Molecularweight	on SDS-PAGE:
	calculated:

Reference: (15)

References:

1. Bunt Milam, A.H. and Saari, J.C. Immunocytochemical localization of two retinoid-binding proteins in vertebrate retina. 1983; J.Cell Biol. 97: 703 - 712.
Goto Top Link to PudMed

2. Chang, M.A., Kennedy, B.N., Crabb, J.W., Hackett, S., Campochiaro, P., and Zack, D.J. Mobility shift assay of human CRAlBP promoter. 1995; Invest.Ophthalmol.Vis.Sci. Suppl. S124
Goto Top

3. Chen, Y., Goldflam, S., Johnson, C., West, K., and Crabb, J.W. The CRAlBP Retinoid Binding Domain. 1993; Invest.Ophthalmol.Vis.Sci. 34: 1198
Goto Top

4. Cotran, P.R., Ringens, P.J., Crabb, J.W., Berson, E.L., and Dryja, T.P. Analysis of the DNA of patients with retinitis pigmentosa with a cellular retinaldehyde binding protein cDNA. 1990; Exp.Eye Res. 51: 15 - 19.
Goto Top Link to PudMed

5. Crabb, J.W., Carlson, A., Chen, Y., Goldflam, S., Intres, R., West, K.A., Hulmes, J.D., Kapron, J.T., Luck, L.A., Horwitz, J., and Bok, D. Structural and functional characterization of recombinant human cellular retinaldehyde-binding protein. 1998; Protein Sci. 7: 746 - 757.
Goto Top Link to PudMed

6. Crabb, J.W., Gaur, V.P., Garwin, G.G., Marx, S.V., Chapline, C., Johnson, C.M., and Saari, J.C. Topological and epitope mapping of the cellular retinaldehyde-binding protein from retina. 1991; J.Biol.Chem. 266: 16674 - 16683.
Goto Top Link to PudMed

7. Crabb, J.W., Johnson, C.M., Carr, S.A., Armes, L.G., and Saari, J.C. The complete primary structure of the cellular retinaldehyde-binding protein from bovine retina. 1988; J.Biol.Chem. 263: 18678 - 18687.
Goto Top Link to PudMed

8. Eisenfeld, A.J., Bunt Milam, A.H., and Saari, J.C. Localization of retinoid-binding proteins in developing rat retina. 1985; Exp.Eye Res. 41: 299 - 304.
Goto Top Link to PudMed

9. Intres, R. Isolation of a mouse retinal cDNA clone encoding CRAlBP. 1994; Invest.Ophthalmol.Vis.Sci. 35: 1464
Goto Top

10. Jones, S.E., Jomary, C., and Neal, M.J. Expression of TIMP3 mRNA is elevated in retinas affected by simplex retinitis pigmentosa. 1994; FEBS Letters. 352: 171 - 174.
Goto Top

11. Kennedy, B.N., Goldman, S., Chang, M.A., Hackett, S., Campochiaro, P., Davis, A., Zack, D.J., and Crabb, J.W. Promotor Analysis of the Human CRALBP Gene. 1995; Invest.Ophthalmol.Vis.Sci. 36: S124
Goto Top

12. Kennedy, B.N., Huang, J., Saari, J.C., and Crabb, J.W. Molecular characterization of the mouse gene encoding cellular retinaldehyde-binding protein. 1998; Mol.Vis. 4:14: 14
Goto Top Link to PudMed

13. Matragoon, S., Chen, H.Y., Traumbauer, M.E., and Liou, G.I. Ribozyme mediated attenuation of interphotoreceptor retinoid binding protein (IRBP) expression results in impaired retinal function. 1995; Invest.Ophthalmol.Vis.Sci. Suppl. S123
Goto Top

14. Maw, M.A., Kennedy, B., Knight, A., Bridges, R., Roth, K.E., Mani, E.J., Mukkadan, J.K., Nancarrow, D., Crabb, J.W., and Denton, M.J. Mutation of the gene encoding cellular retinaldehyde-binding protein in autosomal recessive retinitis pigmentosa. 1997; Nat.Genet. 17: 198 - 200.
Goto Top Link to PudMed

15. Ortego, J., Kennedy, B.N., Crabb, J.W., and Coca-Prados, M. CRAlBP Gene Regulation in Ciliary Epithelial Cells. 1998; Invest.Ophthalmol.Vis.Sci. 39: S39
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16. Pfeffer, B., Wiggert, B., Lee, L., Zonnenberg, B., Newsome, D., and Chader, G. The presence of a soluble interphotoreceptor retinol-binding protein (IRBP) in the retinal interphotoreceptor space. 1983; J.Cell Physiol. 117: 333 - 341.
Goto Top Link to PudMed

17. Saari, J.C. and Bredberg, D.L. Purification of cellular retinaldehyde-binding protein from bovine retina and retinal pigment epithelium. 1988; Exp.Eye Res. 46: 569 - 578.
Goto Top

18. Saari, J.C., Bredberg, L., and Garwin, G.G. Identification of the endogenous retinoids associated with three cellular retinoid-binding proteins from bovine retina and retinal pigment epithelium. 1982; J.Biol.Chem. 257: 13329 - 13333.
Goto Top

19. Saari, J.C., Huang, J., Champer, R.J., Kennedy, B.N., Goldflam, S., and Crabb, J.W. Cloning of the mouse gene encoding cellular retinaldehyde binding protein (CRALBP). 1995; Invest.Ophthalmol.Vis.Sci. 36: S125
Goto Top

20. Sparkes, R.S., Heinzmann, C., Goldflam, S., Kojis, T., Saari, J.C., Mohandas, T., Klisak, I., Bateman, J.B., and Crabb, J.W. Assignment of the Gene (RLBP1) for Cellular Retinaldehyde-Binding Protein (CRALBP) to Human Chromosome-15q26 and Mouse Chromosome-7. 1992; Genomics. 12: 58 - 62.
Goto Top Link to PudMed

21. Yuan, C.C., Crabb, J.W., Fishman, G.A., Jacobson, S.G., and Musarella, M.A. Exclusion of Cellular Retinaldehyde-binding Protein (CRAlBP) as a candidate gene for Stargardt's disease. 1994; Invest.Ophthalmol.Vis.Sci. 35: 1788
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Contact the editor: irpamp@irpa.org	This site is maintained and edited by Dr. rer. medic. Markus Preising, Dipl.Biol. Molecular Genetics Laboratory Department of Paediatric Ophthalmology, Strabismology and Ophthalmogenetics University of Regensburg Head: Prof. Dr. med. Birgit Lorenz